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PubMed · 11354480

Semipreparative capillary electrochromatography.

J R Chen·R N Zare·E C Peters·F Svec·J J Frechét
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Abstract

Capillaries with inner diameters of 550 microm have successfully been packed with 1.5-microm octadecyl silica particles using frits made of macroporous polymers by the UV photopolymerization of a solution of glycidyl methacrylate and trimethylolpropane trimethacrylate. This type of frit is found superior to one made of low-melting point poly(styrene-co-divinylbenzene) beads. Bubble formation is not observed to occur within these capillary columns under our experimental conditions. Separations can be achieved with sample injection volumes as high as 1 microL. To demonstrate its semipreparative use, a mixture of 500 nL of taxol (20 mM) and its precursor, baccatin III (30 mM), is separated using such a column with a Tris buffer.

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AlkaloidsChromatography, High Pressure LiquidElectrochemistryElectrophoresis, CapillaryMethacrylatesPaclitaxelPolymersSilicon DioxideTaxoidsJournal ArticleResearch Support, Non-U.S. Gov't

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J R Chen, R N Zare, E C Peters, F Svec, J J Frechét. 2001-05-01. Semipreparative capillary electrochromatography.. https://doi.org/10.1021/ac001302m

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