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Copper(II)-lincomycin: complexation pattern and oxidative activity.

M Jezowska-Bojczuk·W Lesniak·W Szczepanik·K Gatner·A Jezierski·M Smoluch·W Bal
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Abstract

Coordination of Cu(II) to lincomycin was studied by potentiometry, UV-Vis, circular dichroism (CD), EPR, NMR, cyclic voltammetry (CV) and ESI-MS. Only mononuclear complexes of stoichiometries ranging from CuL to CuH(-3)L were found. In the main species present at neutral pH, CuH(-2)L, lincomycin bonds Cu(II) through both of its nitrogen donors, and a deprotonated oxygen donor at C4 of the sugar moiety. High pressure liquid chromatography (HPLC) of products of 2'-deoxyguanosine (dG) oxidation and agarose gel electrophoresis of plasmid DNA confirmed that lincomycin complexes effectively facilitate dG oxidation by H2O2, but are not able to cleave double-stranded plasmid DNA.

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Anti-Bacterial AgentsChromatography, High Pressure LiquidCopperDeoxyguanosineElectrochemistryHydrogen PeroxideHydrogen-Ion ConcentrationLigandsLincomycinMolecular StructureOxidation-ReductionPlasmidsSpectrum AnalysisTitrimetryJournal ArticleResearch Support, Non-U.S. Gov't

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M Jezowska-Bojczuk, W Lesniak, W Szczepanik, K Gatner, A Jezierski, M Smoluch, W Bal. 2001. Copper(II)-lincomycin: complexation pattern and oxidative activity.. https://doi.org/10.1016/s0162-0134(01)00167-2

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