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PubMed · 11374868

Glycolipids support E-selectin-specific strong cell tethering under flow.

M M Burdick·B S Bochner·B E Collins·R L Schnaar·K Konstantopoulos
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Abstract

This study provides functional evidence that glycosphingolipids constitute ligands for E-selectin but not P-selectin. Chinese hamster ovary (CHO) cells expressing E-selectin (CHO-E) or P-selectin (CHO-P) were perfused over alpha2,3-sialyl Lewis X (alpha2,3-sLe(x)) presented as the hexaosylceramide glycosphingolipid adsorbed in a monolayer containing phosphatidylcholine and cholesterol. CHO-E cells tethered extensively and formed slow, stable rolling interactions with alpha2,3-sLe(x) glycosphingolipid but not with the comparable alpha2,6-sLe(x) glycosphingolipid. Tethering/rolling varied with wall shear stress, selectin density, and ligand density. In contrast, alpha2,3-sLe(x) glycosphingolipid supported only limited, fast CHO-P cell rolling. As calculated from a stochastic model of cell rolling, the step size between successive bond releases from the alpha2,3-sLe(x) glycosphingolipid was smaller for CHO-E than CHO-P cells, whereas the opposite effect was observed for the waiting time between these events. Detachment assays revealed stronger adhesive interactions of CHO-E than CHO-P cells with alpha2,3-sLe(x) glycosphingolipid. These findings indicate that glycosphingolipids expressing an appropriate oligosaccharide mediate cell tethering/rolling via E-selectin but not P-selectin.

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AnimalsCHO CellsCOS CellsCell AdhesionCell ExtractsCell LineCell MovementCricetinaeDose-Response Relationship, DrugE-SelectinEpitopesGlycosphingolipidsHumansLewis X AntigenLigandsLipidsModels, BiologicalNeutrophilsP-SelectinStochastic ProcessesStress, MechanicalTransfectionComparative StudyJournal ArticleResearch Support, Non-U.S. Gov'tResearch Support, U.S. Gov't, Non-P.H.S.Research Support, U.S. Gov't, P.H.S.

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M M Burdick, B S Bochner, B E Collins, R L Schnaar, K Konstantopoulos. 2001-06-01. Glycolipids support E-selectin-specific strong cell tethering under flow.. https://doi.org/10.1006/bbrc.2001.4899

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