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An octakis-intercalating molecule.

M M Murr·M T Harting·V Guelev·J Ren·J B Chaires·B L Iverson
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Abstract

Herein we report the synthesis and characterization of a polyintercalator with eight potential intercalating l,4,5,8-naphthalenetetracarboxylic diimide (NDI) units linked in a head-to-tail arrangement via a peptide linker. UV spectroscopy and viscometry measurements indicated the molecule binds to double-stranded DNA with all eight NDI units intercalated simultaneously. Competition dialysis and DNAse 1 footprinting studies revealed a preference for GC-rich regions of DNA, and circular dichroism studies revealed significant distortion of B-form DNA upon binding. Our so-called "octamer" represents, to the best of our knowledge, the first intercalator that binds as an octakis-intercalator, capable of spanning at least 16 base pairs of DNA.

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AnimalsBase PairingBinding SitesCattleCircular DichroismDNADNA FootprintingGC Rich SequenceImidesIntercalating AgentsNaphthalenesPeptidesSpectrophotometry, UltravioletViscosityJournal ArticleResearch Support, Non-U.S. Gov'tResearch Support, U.S. Gov't, P.H.S.

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M M Murr, M T Harting, V Guelev, J Ren, J B Chaires, B L Iverson. 2001. An octakis-intercalating molecule.. https://doi.org/10.1016/s0968-0896(00)00335-7

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