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Increased throughput in quantitative bioanalysis using parallel-column liquid chromatography with mass spectrometric detection.

M Jemal·M Huang·Y Mao·D Whigan·M L Powell
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Abstract

The feasibility of quantitative bioanalysis by parallel-column liquid chromatography in conjunction with a conventional single-source electrospray mass spectrometer has been investigated using plasma samples containing a drug and its three metabolites. Within a single chromatographic run time, sample injections were made alternately onto each of two analytical columns in parallel at specified intervals, with a mass spectrometer data file opened at every injection. Thus, the mass spectrometer collected data from two sample injections into separate data files within a single chromatographic run time. Therefore, without sacrificing the chromatographic separation or the selected reaction monitoring (SRM) dwell time, the sample throughput was increased by a factor of two. Comparing the method validation results obtained using the two-column system with those obtained using the corresponding conventional single-column approach, the methods on the two systems were found to be equivalent in terms of accuracy and precision. The parallel-column system is simple and can be implemented using existing laboratory equipment with no additional capital outlays. A parallel-column system configured in this manner can be used not only for the within-a-run analysis of two samples containing two different sets of chemical entities, but also for the within-a-run analysis of two samples containing the same set of chemical entities.

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Chromatography, LiquidPiperazinesReproducibility of ResultsSpectrometry, Mass, Electrospray IonizationTime FactorsTriazolesJournal ArticleValidation Study

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M Jemal, M Huang, Y Mao, D Whigan, M L Powell. 2001. Increased throughput in quantitative bioanalysis using parallel-column liquid chromatography with mass spectrometric detection.. https://doi.org/10.1002/rcm.330

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