Conditional gene knockout using Cre recombinase.

Y Le; B Sauer

doi:10.1385/mb:17:3:269

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Conditional gene knockout using Cre recombinase.

Y Le·B Sauer

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Abstract

Cre recombinase has become an important instrument for achieving precise genetic manipulation in mice. Many of these desired genetic manipulations rely on Cre's ability to direct spatially and temporally specified excision of a predesignated DNA sequence that has been flanked by directly repeated copies of the loxP recombination site. Success in achieving such conditional mutagenesis in mice depends both on the careful design of conditional alleles and on reliable detection of cre gene expression. These procedures include PCR, immunohistochemistry and the use of a recombination-proficient GFP-tagged Cre protein.

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Animals Base Sequence Cell Separation Flow Cytometry Genetic Techniques Immunohistochemistry Integrases Mice Mice, Knockout Mice, Transgenic Molecular Sequence Data Polymerase Chain Reaction Recombinant Fusion Proteins Recombination, Genetic Stem Cells Transgenes Viral Proteins Journal Article

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Y Le, B Sauer. 2001. Conditional gene knockout using Cre recombinase.. https://doi.org/10.1385/mb%3A17%3A3%3A269

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