[Study on interactions between TPO molecules tested with yeast two-hybrid system].

Abstract

OBJECTIVE: To explore the interactions between TPO molecules and their locations. METHODS: Full-length TPO, TPO(N) and TPO(C) were inserted respectively into the plasmids of the yeast two-hybrid system. Six different recombinant plasmids were generated. Every 2 recombinant plasmids were transformed into the yeast cell SFY526. The possible interactions between TPOs were then tested by the bioactivity of beta-galactosidase. RESULTS: There are interactions between full-length TPO themselves TPO(N) themselves and TPO with TPO(N), but not between TPO(C)themselves, TPO with TPO(C) and TPO(N) with TPO(C). CONCLUSION: Naturally, the molecular interaction domain of TPO may exist in the N terminus but not in the C terminus.