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Lineage commitment and maturation induction in normal and leukemic preprogenitor cells.

D Metcalf·Y Laâbi
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Abstract

Analysis of the critical cellular processes of self-generation, commitment, and maturation induction ideally requires the use of clonal cultures using cells with a capacity to undergo all three processes. Preprogenitor cells from normal mouse marrow are proving useful cells for such studies. Cells of a newly established cloned leukemic cell line, the GB2, are providing a useful analogous leukemic system because GB2 cells form stratified subpopulations of clonogenic cells able to be clonally analyzed in vitro in which self-renewal is demonstrable, but in which near-normal maturation can be induced by a wide range of hematopoietic regulators.

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AnimalsBone Marrow CellsCell DifferentiationCell LineageCells, CulturedClone CellsColony-Forming Units AssayCulture Media, ConditionedHematopoietic Stem CellsInterleukin-3Leukemia, ExperimentalLymphocytesMacrophage Colony-Stimulating FactorMembrane ProteinsMiceMice, Inbred C57BLMyeloid CellsNeoplastic Stem CellsStem Cell FactorTumor Cells, CulturedTumor Stem Cell AssayWhole-Body IrradiationComparative StudyJournal ArticleResearch Support, Non-U.S. Gov'tResearch Support, U.S. Gov't, P.H.S.

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D Metcalf, Y Laâbi. 2001. Lineage commitment and maturation induction in normal and leukemic preprogenitor cells.. https://doi.org/10.1111/j.1749-6632.2001.tb03597.x

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