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PubMed · 11471730

Copper staining method for extracting biologically active proteins from native gels.

J Wen·M Zhang·T P Horan·J S Philo·T Li·J Wypych·E A Mendiaz·K E Langley·K H Aoki·M Kuwamoto·Y Kita·T Arakawa
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Abstract

An attempt was made to make protein bands visible on native gel using copper staining, since such a mild staining procedure would make the entire native gel electrophoresis process non-denaturing. Copper staining not only was able to detect various proteins on native gel with reasonable sensitivity, but also made extraction and recovery of active proteins possible from the gel using a gentle procedure.

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Chromatography, GelCopperElectrophoresis, Polyacrylamide GelGelsProteinsStaining and LabelingJournal Article

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J Wen, M Zhang, T P Horan, J S Philo, T Li, J Wypych, E A Mendiaz, K E Langley, K H Aoki, M Kuwamoto, Y Kita, T Arakawa. 2001. Copper staining method for extracting biologically active proteins from native gels.. https://doi.org/10.1271/bbb.65.1315

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