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PubMed · 11484604

[Human thymic dendritic cells].

A H Dalloul
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Abstract

Human thymic dendritic cells (DC) were enriched from thymocyte suspension by low density fractionation and elimination of CD3, CD8, CD19, CD56 and CD34-positive cells. Flow cytometry analysis shows that they belong to two distinct populations. The prominent (2/3) one is CD4+ CD45RA+ CD33-CD11c-, mostly immature as it lacks CD80, CD83 and CD86, and is HLA-DRint. High expression of CD123 and expression of pre-TCR-alpha transcripts links this subset to lymphoid, interferon-producing cells. The other DC are typically CD4+ CD33+ CD11c+ myeloid cells mostly mature CD83+ HLA-DRhi. Both subsets could be generated in vitro from thymic CD34+ progenitors. Finally although experimental evidence ascribed to thymic DC a major role in establishing central tolerance through deletion of self reactive thymocytes, the respective functions of human lymphoid and myeloid subsets in the human thymus remain to be established.

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Antigens, CDDendritic CellsFlow CytometryHumansT-LymphocytesThymus GlandEnglish AbstractJournal Article

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A H Dalloul. 2001. [Human thymic dendritic cells].. https://doi.org/10.1016/s0369-8114(01)00164-x

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