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Correlation between histone lysine methylation and developmental changes at the chicken beta-globin locus.

M D Litt·M Simpson·M Gaszner·C D Allis·G Felsenfeld
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Abstract

Methylation of histones at specific residues plays an important role in transcriptional regulation. Chromatin immunoprecipitation of dimethylated lysine 9 on histone H3 across 53 kilobases of the chicken beta-globin locus during erythropoiesis shows an almost complete anticorrelation between regions of elevated lysine 9 methylation and acetylation. Lysine 9 is methylated most over constitutive condensed chromatin and developmentally inactive globin genes. In contrast, lysine 4 methylation of histone H3 correlates with H3 acetylation. These results lead us to propose a mechanism by which the insulator in the beta-globin locus can protect the globin genes from being silenced by adjacent condensed chromatin.

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AcetylationAnimalsAvian ProteinsBrainCarrier ProteinsChick EmbryoChromatinErythrocytesErythroid Precursor CellsErythropoiesisFolate Receptors, GPI-AnchoredGene Expression Regulation, DevelopmentalGene SilencingGlobinsHistonesLocus Control RegionLysineMembrane ProteinsMethylationReceptors, Cell SurfaceReceptors, OdorantTranscriptional ActivationJournal Article

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M D Litt, M Simpson, M Gaszner, C D Allis, G Felsenfeld. 2001-08-09. Correlation between histone lysine methylation and developmental changes at the chicken beta-globin locus.. https://doi.org/10.1126/science.1064413

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