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PubMed · 11509734

Immunocyte Ca2+ influx system mediated by LTRPC2.

Y Sano·K Inamura·A Miyake·S Mochizuki·H Yokoi·H Matsushime·K Furuichi
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Abstract

We characterized an activation mechanism of the human LTRPC2 protein, a member of the transient receptor potential family of ion channels, and demonstrated that LTRPC2 mediates Ca2+ influx into immunocytes. Intracellular pyrimidine nucleotides, adenosine 5'-diphosphoribose (ADPR), and nicotinamide adenine dinucleotide (NAD), directly activated LTRPC2, which functioned as a Ca2+-permeable nonselective cation channel and enabled Ca2+ influx into cells. This activation was suppressed by intracellular adenosine triphosphate. These results reveal that ADPR and NAD act as intracellular messengers and may have an important role in Ca2+ influx by activating LTRPC2 in immunocytes.

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ADP-ribosyl CyclaseADP-ribosyl Cyclase 1Adenosine Diphosphate RiboseAdenosine TriphosphateAntigens, CDAntigens, DifferentiationApoptosisCalciumCalcium ChannelsCell LineEosinophilsHumansIon ChannelsJurkat CellsMembrane GlycoproteinsMembrane PotentialsMembrane ProteinsMonocytesNADNAD+ NucleosidasePatch-Clamp TechniquesReverse Transcriptase Polymerase Chain ReactionT-LymphocytesTRPM Cation ChannelsJournal Article

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Y Sano, K Inamura, A Miyake, S Mochizuki, H Yokoi, H Matsushime, K Furuichi. 2001-08-17. Immunocyte Ca2+ influx system mediated by LTRPC2.. https://doi.org/10.1126/science.1062473

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