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PubMed · 1151746

Immunopharmacological studies using 5-hydroxytryptamine antibody.

R A O'Brien·M Boublik·S Spector
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Abstract

An antibody to serotonin (5-HT) was tested on several preparations in which 5-HT is considered to be involved. The antibody was capable of effectively competing with 5-HT receptor sites for free molecules of 5-HT. It inhibited the uptake of 3H-5HT by blood platelets and may explain the reduced endogenous 5-HT concentration in blood platelets of immunized rabbits. The decreased platelet content of 5-HT was substantiated by electron microscopic studies which showed a reduction in the number of 5-HT-containing subcellular storage organelles in platelets from these animals. The 5-HT antibody inhibited the 5-HT-induced contraction of aortic strips in a tissue bath preparation. When given intraventricularly, it inhibited for a time the sedative actions of reserpine thus adding new evidence to the often postulated role of 5-HT in the actions of reserpine.

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Adenylyl CyclasesAnimalsAntibodiesBinding, CompetitiveBlood PlateletsDose-Response Relationship, DrugMaleMicroscopy, ElectronMyocardial ContractionRabbitsRatsReserpineSerotoninJournal Article

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R A O'Brien, M Boublik, S Spector. 1975. Immunopharmacological studies using 5-hydroxytryptamine antibody.. https://pubmed.ncbi.nlm.nih.gov/1151746/

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