Gene expression profiling in human esophageal cancers using cDNA microarray.

T Kan; Y Shimada; F Sato; M Maeda; A Kawabe; J Kaganoi; A Itami; S Yamasaki; M Imamura

doi:10.1006/bbrc.2001.5400

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Gene expression profiling in human esophageal cancers using cDNA microarray.

T Kan·Y Shimada·F Sato·M Maeda·A Kawabe·J Kaganoi·A Itami·S Yamasaki·M Imamura

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Abstract

Human esophageal cancer cell lines and human esophageal cancer tissues were profiled on cDNA microarrays. In esophageal cancer cell lines, KYAE and OE-33 (adenocarcinomas) were distinguished from KYSE series (squamous cell carcinomas). Although SK-GT-4 and TE7 were derived from adenocarcinomas, they had a comparatively similar expression profile to the KYSE series. A set of genes whose expression commonly either increased or decreased in cancer cell lines was identified. Genes that were characteristically expressed in KYAE and OE-33 were also identified. The gene expression profiles of cancer tissues (CTs) were remarkably different from those of the cancer cell lines (CCLs). Notable differences between CCLs and CTs were observed in matrix metalloproteinases, plasminogen activator, collagens, paxillin, and thrombospondin 2, etc., whose expression was not increased in CCLs but increased in CTs. Twenty-three genes were extracted to categorize patients according to their prognoses, and clustering analyses, using these genes, were performed successfully.

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Adenocarcinoma Carcinoma, Squamous Cell Down-Regulation Esophageal Neoplasms Gene Expression Profiling Humans Neoplasm Proteins Oligonucleotide Array Sequence Analysis Polymerase Chain Reaction RNA, Neoplasm Receptors, Cytoplasmic and Nuclear Transcription Factors Tumor Cells, Cultured Up-Regulation Journal Article

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T Kan, Y Shimada, F Sato, M Maeda, A Kawabe, J Kaganoi, A Itami, S Yamasaki, M Imamura. 2001-08-31. Gene expression profiling in human esophageal cancers using cDNA microarray.. https://doi.org/10.1006/bbrc.2001.5400

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