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PubMed · 11544049

General purpose, field-portable cell-based biosensor platform.

K H Gilchrist·V N Barker·L E Fletcher·B D DeBusschere·P Ghanouni·L Giovangrandi·G T Kovacs
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Abstract

There are several groups of researchers developing cell-based biosensors for chemical and biological warfare agents based on electrophysiologic monitoring of cells. In order to transition such sensors from the laboratory to the field, a general-purpose hardware and software platform is required. This paper describes the design, implementation, and field-testing of such a system, consisting of cell-transport and data acquisition instruments. The cell-transport module is a self-contained, battery-powered instrument that allows various types of cell-based modules to be maintained at a preset temperature and ambient CO(2) level while in transit or in the field. The data acquisition module provides 32 channels of action potential amplification, filtering, and real-time data streaming to a laptop computer. At present, detailed analysis of the data acquired is carried out off-line, but sufficient computing power is available in the data acquisition module to enable the most useful algorithms to eventually be run real-time in the field. Both modules have sufficient internal power to permit realistic field-testing, such as the example presented in this paper.

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Action PotentialsAlgorithmsAnimalsBiosensing TechniquesCell LineEquipment DesignMiceMyocardiumSoftwareEvaluation StudyJournal ArticleResearch Support, U.S. Gov't, Non-P.H.S.Validation Study

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K H Gilchrist, V N Barker, L E Fletcher, B D DeBusschere, P Ghanouni, L Giovangrandi, G T Kovacs. 2001. General purpose, field-portable cell-based biosensor platform.. https://doi.org/10.1016/s0956-5663(01)00169-5

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