Mapping protein-protein interactions with combinatorial biology methods.

J Pelletier; S Sidhu

doi:10.1016/s0958-1669(00)00225-1

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Mapping protein-protein interactions with combinatorial biology methods.

J Pelletier·S Sidhu

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Abstract

Extremely diverse, DNA-encoded libraries of peptides and proteins have been constructed that include a linkage between each polypeptide and the encoding DNA. Library members can be selected by virtue of a particular binding specificity, and their protein sequence can be deduced from the sequence of the cognate DNA. Such combinatorial biology methods have proven invaluable in both identifying natural protein-protein interactions and also in mapping the specificities and energetics of these interactions in fine detail.

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Amino Acid Sequence Base Sequence Combinatorial Chemistry Techniques DNA Peptide Library Protein Binding Proteins Sensitivity and Specificity Journal Article Review

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J Pelletier, S Sidhu. 2001. Mapping protein-protein interactions with combinatorial biology methods.. https://doi.org/10.1016/s0958-1669(00)00225-1

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