Preparation of oligodeoxynucleotide 5'-triphosphates using solid support approach.

A V Lebedev; I I Koukhareva; T Beck; M M Vaghefi

doi:10.1081/ncn-100002565

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Preparation of oligodeoxynucleotide 5'-triphosphates using solid support approach.

A V Lebedev·I I Koukhareva·T Beck·M M Vaghefi

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Abstract

We report a synthetic procedure for conversion of oligonucleotides to their 5'-triphosphate derivatives with moderate yield. The oligonucleotides were synthesized on solid support using standard phosphoramidite protocols. The DMT protection group was removed and the 5'-OH was phosphitylated using 2-chloro-4H-1,3,2-benzodioxaphosphorin-4-one followed by reaction with tributyammonium pyrophosphate and iodine oxidation. After subsequent removal from support and complete deprotection, the products were isolated by anion-exchange HPLC chromatography. Structures of several 5'-triphosphate derivatives have been proven by phosphorus NMR, Mass-spectrometry and by HPLC comparison with authentic samples.

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Chromatography, High Pressure Liquid Nuclear Magnetic Resonance, Biomolecular Oligonucleotides Organophosphates Organophosphorus Compounds Journal Article

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A V Lebedev, I I Koukhareva, T Beck, M M Vaghefi. Preparation of oligodeoxynucleotide 5'-triphosphates using solid support approach.. https://doi.org/10.1081/ncn-100002565

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