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PubMed · 11563070

High capacity substrates as a platform for a DNA probe array genotyping assay.

J Fidanza·M Glazer·D Mutnick·G McGall·C Frank
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Abstract

Colloidal silica particles were deposited on a glass substrate to produce high-capacity porous supports for high-density DNA probe arrays. Porous surfaces were used to increase the addressable surface area and number of probes available for hybridization. Surfaces derived from 70-100 nm size particles deposited in films from 0.15 to 2 microns thick exhibited excellent performance in light-directed oligonucleotide synthesis. Evaluation of these substrates in a genotyping assay is reported.

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ColloidsDNA ProbesDNA, ViralGenotypeGlassHIVNucleic Acid HybridizationOligonucleotide Array Sequence AnalysisParticle SizeSilicon DioxideSurface PropertiesJournal Article

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J Fidanza, M Glazer, D Mutnick, G McGall, C Frank. High capacity substrates as a platform for a DNA probe array genotyping assay.. https://doi.org/10.1081/ncn-100002329

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