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PubMed · 11576725

Using MF-PCR to diagnose multiple defects from single cells: implications for PGD.

I Findlay·P L Matthews·B K Mulcahy·K Mitchelson
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Abstract

Single cell genetic analysis is generally performed using PCR and FISH. Until recently, FISH has been the method of choice. FISH however is expensive, has significant misdiagnosis rates, can result in interpretation difficulties and is labour intensive making it unsuitable for high throughput processing. Recently fluorescent PCR reliability has increased to levels at or surpassing FISH whilst maintaining low cost. However, PCR accuracy has been a concern due to allelic dropout. Multiplex PCR can now increase accuracy by using multiple markers for each chromosome to firstly provide diagnosis if markers fail and/or secondly confirm diagnosis. We compare a variety of diagnostic methods and demonstrate for the first time a multiplex PCR system providing simultaneous diagnosis and confirmation of the major aneuploidy chromosomes (21,18,13) and sex as well as DNA fingerprint in single cells. We also discuss the implications of using PCR for aneuploidy screening in preimplantation genetic diagnosis.

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AneuploidyBlastomeresChromosomesCystic FibrosisDNA FingerprintingFemaleFetal DiseasesFluorescent DyesHumansIn Situ Hybridization, FluorescenceKaryotypingPolymerase Chain ReactionPregnancyPreimplantation DiagnosisReproducibility of ResultsSensitivity and SpecificitySex Determination ProcessesTrisomyComparative StudyJournal Article

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I Findlay, P L Matthews, B K Mulcahy, K Mitchelson. 2001-10-22. Using MF-PCR to diagnose multiple defects from single cells: implications for PGD.. https://doi.org/10.1016/s0303-7207(01)00567-6

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