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UV absorption method should not be applied for determining amoxycillin in acidic dissolution test medium.

T Tokumura·Y Machida
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Abstract

In order to clarify the suitability of assay method, the stability of amoxycillin (AMX) in the acidic solution at pH 1.2 was determined by two different methods, ultraviolet absorption (UV) and HPLC methods. The determination was simultaneously performed for the same sample solutions. The data from HPLC method indicated that AMX degraded and the concentration of AMX in the acidic solution was decreased. The apparent first-order rate constant (k(obs)) for the degradation was 9.83x10(-2) h(-1), and the half-life was calculated to be 7.04 h. On the other hand, the data obtained from UV method apparently represented that the concentration of AMX increased with time-course. This phenomena was brought about by the increase of UV absorption at 272 nm, which accompanied with the degradation of AMX. These results clearly indicate that UV method can not be used for determining the samples accompanying the degradation of AMX. In general, the dissolution test (drug release test) has been used for the evaluation of a sustained-release dosage form of AMX. It is impossible to avoid the degradation of AMX in the test under acidic conditions. The results indicated that the UV method should not be used for the determination of AMX in the test.

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AcidsAmoxicillinChromatography, High Pressure LiquidDrug Delivery SystemsPenicillinsSolubilitySpectrophotometry, UltravioletJournal Article

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BibTeXRIS

T Tokumura, Y Machida. 2001-10-09. UV absorption method should not be applied for determining amoxycillin in acidic dissolution test medium.. https://doi.org/10.1016/s0378-5173(01)00837-7

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