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Strand-specific postreplicative processing of mammalian telomeres.

S M Bailey·M N Cornforth·A Kurimasa·D J Chen·E H Goodwin
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Abstract

Telomeres are specialized nucleoprotein structures that stabilize the ends of linear eukaryotic chromosomes. In mammalian cells, abrogation of telomeric repeat binding factor TRF2 or DNA-dependent protein kinase (DNA-PK) activity causes end-to-end chromosomal fusion, thus establishing an essential role for these proteins in telomere function. Here we show that TRF2-mediated end-capping occurs after telomere replication. The postreplicative requirement for TRF2 and DNA-PKcs, the catalytic subunit of DNA-PK, is confined to only half of the telomeres, namely, those that were produced by leading-strand DNA synthesis. These results demonstrate a crucial difference in postreplicative processing of telomeres that is linked to their mode of replication.

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AnimalsCell DivisionCell LineChromatidsChromosomesDNA ReplicationDNA-Activated Protein KinaseDNA-Binding ProteinsHumansIn Situ HybridizationMiceMitosisMutationNuclear ProteinsProtein Serine-Threonine KinasesTelomereTelomeric Repeat Binding Protein 2Tumor Cells, CulturedNon-programmaticJournal ArticleResearch Support, U.S. Gov't, Non-P.H.S.Research Support, U.S. Gov't, P.H.S.

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S M Bailey, M N Cornforth, A Kurimasa, D J Chen, E H Goodwin. 2001-09-28. Strand-specific postreplicative processing of mammalian telomeres.. https://doi.org/10.1126/science.1062560

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