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A normalized plot as a novel and time-saving tool in complex enzyme kinetic analysis.

I G Bravo·F Busto·D De Arriaga·M A Ferrero·L B Rodríguez-Aparicio·H Martínez-Blanco·A Reglero
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Abstract

A new data treatment is described for designing kinetic experiments and analysing kinetic results for multi-substrate enzymes. Normalized velocities are plotted against normalized substrate concentrations. Data are grouped into n + 1 families across the range of substrate or product tested, n being the number of substrates plus products assayed. It has the following advantages over traditional methods: (1) it reduces to less than a half the amount of data necessary for a proper description of the system; (2) it introduces a self-consistency checking parameter that ensures the 'scientific reliability' of the mathematical output; (3) it eliminates the need for a prior knowledge of Vmax; (4) the normalization of data allows the use of robust and fuzzy methods suitable for managing really 'noisy' data; (5) it is appropriate for analysing complex systems, as the complete general equation is used, and the actual influence of effectors can be typified; (6) it is amenable to being implemented as a software that incorporates testing and electing among rival kinetic models.

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EnzymesKineticsModels, ChemicalModels, StatisticalComparative StudyJournal ArticleResearch Support, Non-U.S. Gov't

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I G Bravo, F Busto, D De Arriaga, M A Ferrero, L B Rodríguez-Aparicio, H Martínez-Blanco, A Reglero. 2001-09-15. A normalized plot as a novel and time-saving tool in complex enzyme kinetic analysis.. https://doi.org/10.1042/bj3580573

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