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PubMed · 11602048

[Construction of a dicistronic expression plasmid vector containing double-valent hepatitis B surface gene].

B Wang·D Zhang·A Huang·Y Yao·Q Liu·H Ren
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Abstract

OBJECTIVE: In order to enhance vaccine response, we constructed a dicistronic expression plasmid containing double HBsAg immunogenes. METHODS: At first, pcDNA3.1 plasmid vector was digested with NheI and EcoRI to get the coding sequence of the small (S) surface protein of HBV, then cloned into pCI-neo vector and named it pCI-S. By PCR amplification, the product of IRES-S was digested with SalI & BamHI, and cloned into pBluescript IIK+S to generate pBKS-IRES-S vector, then subcloned to the pCI-S plasmid to generate pCI-S-IRES-S, which is a dicistronic plasmid of double value HBsAg genes. RESULTS: Two plasmids we constructed were digested with related restriction nucleic enzymes. Sequence analysis of HBsAg and IRES-S gene did not reveal any mutation. CONCLUSIONS: The construction of dicistronic plasmid of divalue HBsAg immunogenes has been well cloned, which is convenient for further research on cell expression and gene immunization in animals.

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Antigens, ViralGene ExpressionGenetic VectorsHepatitis B Surface AntigensHepatitis B virusPlasmidsPolymerase Chain ReactionRestriction MappingEnglish AbstractJournal Article

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B Wang, D Zhang, A Huang, Y Yao, Q Liu, H Ren. 2001. [Construction of a dicistronic expression plasmid vector containing double-valent hepatitis B surface gene].. https://pubmed.ncbi.nlm.nih.gov/11602048/

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