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PubMed · 11606418

A hybrid bacterial replication origin.

H Seitz·M Welzeck·W Messer
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Abstract

We constructed a hybrid replication origin that consists of the main part of oriC from Escherichia coli, the DnaA box region and the AT-rich region from Bacillus subtilis oriC. The AT-rich region could be unwound by E. coli DnaA protein, and the DnaB helicase was loaded into the single-stranded bubble. The results show that species specificity, i.e. which DnaA protein can do the unwinding, resides within the DnaA box region of oriC.

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Bacillus subtilisBacterial ProteinsBase SequenceDNA FootprintingDNA HelicasesDNA-Binding ProteinsDnaB HelicasesEscherichia coliMolecular Sequence DataPlasmidsPotassium PermanganateReplication OriginSpecies SpecificityJournal ArticleResearch Support, Non-U.S. Gov't

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H Seitz, M Welzeck, W Messer. 2001-10-17. A hybrid bacterial replication origin.. https://doi.org/10.1093/embo-reports%2Fkve225

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