Neurocalcin-actin interaction.

D Mornet; A Bonet-Kerrache

doi:10.1016/s0167-4838(01)00260-6

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PubMed · 11690657

Neurocalcin-actin interaction.

D Mornet·A Bonet-Kerrache

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Abstract

Neurocalcin is an N-myristoylated calcium-binding protein which belongs to a novel family of neuronal calcium sensors. Here we show, by cosedimentation, co-immunoprecipitation and cross-linking approaches, that myristoylated neurocalcin directly interacts with actin in a calcium-dependent manner. We used EDC cross-linking and obtained one novel 64 kDa entity composed of one actin molecule and one neurocalcin molecule, as demonstrated with IAEDANS-actin and neurocalcin-specific antibodies. This interaction could modulate the rod outer segment-guanylate cyclase 1-neurocalcin interface.

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Actins Animals Calcium Calcium-Binding Proteins Cattle Chemical Precipitation Cross-Linking Reagents Dimerization Electrophoresis, Polyacrylamide Gel Nerve Tissue Proteins Neurocalcin Precipitin Tests Rabbits Receptors, Calcium-Sensing Journal Article

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D Mornet, A Bonet-Kerrache. 2001-10-18. Neurocalcin-actin interaction.. https://doi.org/10.1016/s0167-4838(01)00260-6

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