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PubMed · 11699906

Spatial temperature gradient capillary electrophoresis for DNA mutation detection.

L Zhu·H K Lee·B Lin·E S Yeung
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Abstract

A continuous spatial temperature gradient was established in capillary electrophoresis by using a simple temperature control device. The temperature profile along the capillary was predicted by theoretical calculations. A nearly linear spatial temperature gradient was established and applied to DNA mutation detection. By spanning a wide temperature range, it was possible to perform simultaneous heteroduplex analysis for various mutation types that have different melting temperatures.

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DNADNA Mutational AnalysisElectrophoresis, CapillaryIndicators and ReagentsNucleic Acid DenaturationNucleic Acid HeteroduplexesPoint MutationTemperatureThermodynamicsJournal ArticleResearch Support, Non-U.S. Gov'tResearch Support, U.S. Gov't, Non-P.H.S.Research Support, U.S. Gov't, P.H.S.

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L Zhu, H K Lee, B Lin, E S Yeung. 2001. Spatial temperature gradient capillary electrophoresis for DNA mutation detection.. https://doi.org/10.1002/1522-2683(200109)22%3A17%3C3683%3A%3Aaid-elps3683%3E3.0.co%3B2-b

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