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Miniaturized multichannel electrospray ionization emitters on poly(dimethylsiloxane) microfluidic devices.

J S Kim·D R Knapp
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Abstract

A multichannel electrospray ionization (ESI) emitter was fabricated as part of a poly(dimethylsiloxane) (PDMS) microfluidic device using a three-layer photoresist process which also produces a self-alignment system to make a bonding between the top and bottom PDMS parts. The prototype device (2 cm high x 5 cm wide x 5 cm long) had 16-channels (30 microm wide x 50 microm deep) with emitters of 1 mm length and 60 degrees point angle. The PDMS emitter tips enabled interfacing the device to ESI-mass spectrometry; a stable electrospray from the tips was performed with limits of detection under 1 microM for reference peptides (adrenocorticotropic hormone fragment 1-17, angiotensin I and III).

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Adrenocorticotropic HormoneAngiotensin IAngiotensin IIIEquipment DesignHumansMicrochemistryMiniaturizationPeptide FragmentsRheologySpectrometry, Mass, Electrospray IonizationJournal ArticleResearch Support, U.S. Gov't, Non-P.H.S.

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J S Kim, D R Knapp. 2001. Miniaturized multichannel electrospray ionization emitters on poly(dimethylsiloxane) microfluidic devices.. https://doi.org/10.1002/1522-2683(200110)22%3A18%3C3993%3A%3Aaid-elps3993%3E3.0.co%3B2-x

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