Two-photon excitation fluorescence microscopy.

P T So; C Y Dong; B R Masters; K M Berland

doi:10.1146/annurev.bioeng.2.1.399

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Two-photon excitation fluorescence microscopy.

P T So·C Y Dong·B R Masters·K M Berland

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Abstract

Two-photon fluorescence microscopy is one of the most important recent inventions in biological imaging. This technology enables noninvasive study of biological specimens in three dimensions with submicrometer resolution. Two-photon excitation of fluorophores results from the simultaneous absorption of two photons. This excitation process has a number of unique advantages, such as reduced specimen photodamage and enhanced penetration depth. It also produces higher-contrast images and is a novel method to trigger localized photochemical reactions. Two-photon microscopy continues to find an increasing number of applications in biology and medicine.

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Animals Biomedical Engineering Fluorescent Dyes History, 20th Century Humans Image Processing, Computer-Assisted Microscopy, Confocal Microscopy, Fluorescence Microscopy, Video Optics and Photonics Photons Historical Article Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S.Research Support, U.S. Gov't, P.H.S.Review

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P T So, C Y Dong, B R Masters, K M Berland. 2000. Two-photon excitation fluorescence microscopy.. https://doi.org/10.1146/annurev.bioeng.2.1.399

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