Calculated background elimination in quantifying nitric-oxide synthase enzyme activity.

F Stonek; W Tschugguel; J C Huber; C Schneeberger

doi:10.1016/s0165-022x(01)00171-3

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Calculated background elimination in quantifying nitric-oxide synthase enzyme activity.

F Stonek·W Tschugguel·J C Huber·C Schneeberger

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Abstract

Measuring nitric-oxide synthase (NOS) activity by monitoring the conversion of L-arginine to L-citrulline is currently the standard assay for NOS activity. We describe a simple method of quantifying low values of NOS activity by removing the background mathematically. When performing NOS activity studies in samples with low protein amount (< 25 microg/microl), we encountered the problem of sample values that can hardly be differentiated from blank values probably originating from radioactive-labeled arginine in the final eluate. Our method determines mathematically these background values and may be an improvement of the citrulline assay.

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Arginine Biochemistry Citrulline Humans Models, Theoretical Nitric Oxide Synthase Temperature Journal Article Research Support, Non-U.S. Gov't

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F Stonek, W Tschugguel, J C Huber, C Schneeberger. 2001-12-04. Calculated background elimination in quantifying nitric-oxide synthase enzyme activity.. https://doi.org/10.1016/s0165-022x(01)00171-3

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