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Identification of sonic hedgehog-responsive genes using cDNA microarray.

M Kato·N Seki·S Sugano·K Hashimoto·Y Masuho·M Muramatsu·K Kaibuchi·M Nakafuku
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Abstract

Sonic hedgehog (Shh) is a secreted signaling protein that plays important roles in a variety of developmental processes and also in pathogenesis of some human cancers and congenital diseases. Molecules that function downstream of Shh, however, still remain elusive. Here we searched for Shh-responsive genes by using an in-house cDNA microarray. Two genes were newly identified to be Shh responsive in neuroepithelial cell line MNS-70: the metal-binding protein Ceruloplasmin (Cp) and the serine protease inhibitor inter-alpha-trypsine inhibitor heavy chain H3 (ITIH3). In MNS-70 cells, expression of ITIH3 was regulated by Gli zinc-finger transcription factors downstream of Shh, whereas Cp appeared to be regulated by Gli-independent pathways. Cp mRNA was detected in the developing mouse brain, where its expression domain was closely adjacent to that of Shh. These results demonstrate that microarray technology provides a useful tool for studying expression of developmentally regulated genes.

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AnimalsBrainCell LineCeruloplasminDNA, ComplementaryEnzyme InhibitorsGene Expression Regulation, DevelopmentalHedgehog ProteinsHumansIn Situ HybridizationMiceMice, Inbred ICROligonucleotide Array Sequence AnalysisPlasmidsProtein PrecursorsProtein Structure, TertiaryRNA, MessengerReverse Transcriptase Polymerase Chain ReactionSerine EndopeptidasesTrans-ActivatorsTransfectionTrypsin InhibitorsZinc FingersJournal Article

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M Kato, N Seki, S Sugano, K Hashimoto, Y Masuho, M Muramatsu, K Kaibuchi, M Nakafuku. 2001-11-30. Identification of sonic hedgehog-responsive genes using cDNA microarray.. https://doi.org/10.1006/bbrc.2001.5976

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