[Basic study on the sensitivity test for inhibiting fibroblast proliferation (II)].

Y Sato; T Kondo; F Yamada

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PubMed · 11729481

[Basic study on the sensitivity test for inhibiting fibroblast proliferation (II)].

Y Sato·T Kondo·F Yamada

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Abstract

The results of in vitro tests against solid tumors have been generally influenced by the mixing in of fibroblasts, and so have SDI tests. We demonstrated that the influence of fibroblasts can be excluded by performing the SDI test on an agar layer, thus significantly inhibiting the growth of fibroblasts in the liquid top layer. In the agar SDI test, SDS is used to dissolve formazan; however, this lowers the sensitivity to some extent. We added additional DMSO to SDS and to raise the sensitivity.

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Antineoplastic Agents Cisplatin Colonic Neoplasms Doxorubicin Drug Screening Assays, Antitumor Fibroblasts Fluorouracil Humans Mitomycin Stomach Neoplasms Tumor Cells, Cultured English Abstract Journal Article

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Y Sato, T Kondo, F Yamada. 2001. [Basic study on the sensitivity test for inhibiting fibroblast proliferation (II)].. https://pubmed.ncbi.nlm.nih.gov/11729481/

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