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NGF induces neurite outgrowth via a decrease in phosphorylation of myosin light chain in PC12 cells.

A Fujita·Y Hattori·T Takeuchi·Y Kamata·F Hata
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Abstract

The relationship between phosphorylation of myosin light chain (MLC) and neurite outgrowth induced by nerve growth factor (NGF) was studied in PC12 cells. Inhibitors of Rho kinase, HA-1077 or Y-27632 also induced neurite outgrowth. As already reported botulinum exoenzyme C3 which inactivates Rho protein also induced neurite outgrowth. Calyeulin A, an inhibitor of phosphatase counteracted both NGF- and C3- induced neurite outgrowth. Treatments of both NGF and C3 resulted in significant and transient decrease in phosphorylated MLC. These results suggest that NGF induces neurite outgrowth of PC12 by a transient decrease in phosphorylated MLC which is brought about by activation of MLC phosphatase via inhibition of Rho-Rho kinase pathway.

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AnimalsDose-Response Relationship, DrugIntracellular Signaling Peptides and ProteinsMyosin Light ChainsNerve Growth FactorNeuritesPC12 CellsPhosphorylationProtein Serine-Threonine KinasesRatsrho-Associated KinasesJournal Article

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A Fujita, Y Hattori, T Takeuchi, Y Kamata, F Hata. 2001-11-16. NGF induces neurite outgrowth via a decrease in phosphorylation of myosin light chain in PC12 cells.. https://doi.org/10.1097/00001756-200111160-00045

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