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Differential requirement for SLP-76 domains in T cell development and function.

P S Myung·G S Derimanov·M S Jordan·J A Punt·Q H Liu·B A Judd·E E Meyers·C D Sigmund·B D Freedman·G A Koretzky
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Abstract

The hematopoietic cell-specific adaptor protein, SLP-76, is critical for T cell development and mature T cell receptor (TCR) signaling; however, the structural requirements of SLP-76 for mediating thymopoiesis and mature T cell function remain largely unknown. In this study, transgenic mice were generated to examine the requirements for specific domains of SLP-76 in thymocytes and peripheral T cells in vivo. Examination of mice expressing various mutants of SLP-76 on the null background demonstrates a differential requirement for specific domains of SLP-76 in thymocytes and T cells and provides new insight into the molecular mechanisms underlying SLP-76 function.

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Adaptor Proteins, Signal TransducingAmino Acid MotifsAmino Acid SubstitutionAnimalsBinding SitesCD3 ComplexCalcium SignalingCarrier ProteinsCell DifferentiationClonal DeletionImmunophenotypingMembrane ProteinsMiceMice, Inbred C57BLMice, KnockoutMice, TransgenicMutation, MissensePhosphoproteinsProtein Structure, TertiaryReceptors, Antigen, T-CellRecombinant Fusion ProteinsSequence DeletionSignal TransductionSpleenStructure-Activity RelationshipT-LymphocytesThymus Glandsrc Homology DomainsComparative StudyJournal ArticleResearch Support, Non-U.S. Gov'tResearch Support, U.S. Gov't, P.H.S.

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P S Myung, G S Derimanov, M S Jordan, J A Punt, Q H Liu, B A Judd, E E Meyers, C D Sigmund, B D Freedman, G A Koretzky. 2001. Differential requirement for SLP-76 domains in T cell development and function.. https://doi.org/10.1016/s1074-7613(01)00253-9

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