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Introns in protein-coding genes in Archaea.

Yoh-ichi Watanabe·Shin-ichi Yokobori·Toshiro Inaba·Akihiko Yamagishi·Tairo Oshima·Yutaka Kawarabayasi·Hisasi Kikuchi·Kiyoshi Kita
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Abstract

Introns in protein-coding genes are ubiquitous in eukaryotic cells, but pre-mRNA splicing has yet to be reported in archaeal and its viral genomes. We present evidence of introns in genes encoding a homolog of eukaryotic Cbf5p (centromere-binding factor 5; a subunit of a small nucleolar ribonucleoprotein) in three Archaea; Aeropyrum pernix, Sulfolobus solfataricus and Sulfolobus tokodaii. Splicing of pre-mRNAs in vivo was demonstrated by reverse transcriptase-mediated polymerase chain reaction. The exon-intron boundaries of these genes are predicted to be folded into a structure similar to the bulge-helix-bulge motif, suggesting that splicing of these pre-mRNAs probably depends on the splicing system elucidated for archaeal pre-tRNAs and rRNAs.

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Amino Acid SequenceArchaeal ProteinsBase SequenceDNA, ArchaealDesulfurococcaceaeGenes, ArchaealHydro-LyasesIntronsMicrotubule-Associated ProteinsMolecular Sequence DataRNA PrecursorsRNA SplicingRNA-Binding ProteinsRibonucleoproteins, Small NuclearSaccharomyces cerevisiae ProteinsSequence Homology, Amino AcidSulfolobusJournal Article

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Yoh-ichi Watanabe, Shin-ichi Yokobori, Toshiro Inaba, Akihiko Yamagishi, Tairo Oshima, Yutaka Kawarabayasi, Hisasi Kikuchi, Kiyoshi Kita. 2002-01-02. Introns in protein-coding genes in Archaea.. https://doi.org/10.1016/s0014-5793(01)03219-7

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