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PubMed · 11763917

Microscopy investigations. Techniques, results, limitations.

B Van Meerbeek·M Vargas·S Inoue·Y Yoshida·J Perdigão·P Lambrechts·G Vanherle
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Abstract

This paper critically reviews current microscopy techniques that are used to image resin-dentin interfaces and more in particular to investigate the process of hybridization and resin-tag formation. A short description of the four most commonly used microscopy techniques with their necessary specimen-preparation methodology is provided along with a discussion of their possibilities, limitations and potential artifact formation. With this critical appraisal on microscopy techniques available to study adhesive interfaces, it is hoped to encourage the use of high-resolution analytical tools to further elucidate the mechanisms of bonding at the ultra-structural level. Besides ultra-morphologic characterization, more research should be devoted to study the chemical and physical properties of resin-dentin bonds. The final objective of such fundamental research should be to establish a more durable and reliable adhesive restorative technique.

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ArtifactsDental BondingDentinDentin-Bonding AgentsHistocytological Preparation TechniquesHumansMicroscopyMicroscopy, Atomic ForceMicroscopy, ConfocalMicroscopy, ElectronMicroscopy, Electron, ScanningSurface PropertiesJournal ArticleResearch Support, Non-U.S. Gov'tReview

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B Van Meerbeek, M Vargas, S Inoue, Y Yoshida, J Perdigão, P Lambrechts, G Vanherle. 2000. Microscopy investigations. Techniques, results, limitations.. https://pubmed.ncbi.nlm.nih.gov/11763917/

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