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Non-antisense cellular responses to oligonucleotides.

Alain Anselmet·Ebrahim Mayat·Stefan Wietek·Paul G Layer·Bernard Payrastre·Jean Massoulié
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Abstract

Oligonucleotides induce various cellular responses which are not due to the blockade of protein synthesis by an antisense mechanism. Oligonucleotides presenting double-stranded or G-quartet structures (ribo- or deoxyribonucleotides, phosphodiester or phosphorothioated) induce retraction of neurites and aggregation of chicken retinal cells within 10-20 h. This effect is reversible, non-toxic; it appears to require internalization and can be mimicked by treatment of the cells with an RGDS peptide. The oligonucleotides appear to trigger a cascade of intracellular events, affecting the adhesive properties of integrins. In addition, a subset of oligonucleotides induced platelet aggregation, probably through their interaction with membrane receptors. Recognition of these effects is important for the design and interpretation of antisense experiments.

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AnimalsBlood PlateletsCalciumCell AggregationCells, CulturedChick EmbryoHumansIntegrinsIntracellular FluidNeuritesNucleic Acid ConformationOligonucleotidesOligopeptidesPeptidesPlatelet AggregationPlatelet Aggregation InhibitorsRetinaTransfectionJournal ArticleResearch Support, Non-U.S. Gov't

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Alain Anselmet, Ebrahim Mayat, Stefan Wietek, Paul G Layer, Bernard Payrastre, Jean Massoulié. 2002-01-16. Non-antisense cellular responses to oligonucleotides.. https://doi.org/10.1016/s0014-5793(01)03248-3

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