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Electrochemically mediated electrodeposition/electropolymerization to yield a glucose microbiosensor with improved characteristics.

Xiaohong Chen·Norio Matsumoto·Yibai Hu·George S Wilson
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Abstract

A procedure is described that provides for electrochemically mediated deposition of enzyme and a polymer layer permselective for endogenous electroactive species. Electrodeposition was first employed for the direct immobilization of glucose oxidase to produce a uniform, thin, and compact film on a Pt electrode. Electropolymerization of phenol was then employed to form an anti-interference and protective polyphenol film within the enzyme layer. In addition, a stability-reinforcing membrane derived from (3-aminopropyl)trimethoxysilane was constructed by electrochemically assisted cross-linking. This hybrid film outside the enzyme layer contributed to the improved stability and permselectivity. The resulting glucose sensor was characterized by a short response time (<4 s), high sensitivity (1200 nA/mM x cm2), low interference from endogenous electroactive species, and working lifetime of more than 50 days.

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Biosensing TechniquesElectrochemistryElectrodesEnzymes, ImmobilizedGlucoseGlucose OxidaseMiniaturizationPolymersJournal ArticleResearch Support, U.S. Gov't, P.H.S.

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Xiaohong Chen, Norio Matsumoto, Yibai Hu, George S Wilson. 2002-01-15. Electrochemically mediated electrodeposition/electropolymerization to yield a glucose microbiosensor with improved characteristics.. https://doi.org/10.1021/ac015628m

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