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PubMed · 11814805

Dual analyte detection using tandem flash luminescence.

Maciej Adamczyk·Jeffrey A Moore·Kevin Shreder
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Abstract

A heterogeneous, dual analyte-binding assay which makes use of the flash luminescence from both aequorin and an acridinium-9-carboxamide label is presented. The signal generating species were triggered both differentially and sequentially using Ca(2+) followed by basic peroxide. Both signals were resolved readily using a single photomultiplier tube without the need for multiwavelength detection. To demonstrate the tandem luminescence concept in a model assay system, dose-response curves for two analytes, biotinylated BSA and myoglobin, were generated using a competitive binding format. Because of the relatively short assay time and the well-resolved signals, this format will be useful in the development of dual analyte high-throughput assays.

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AcridinesAequorinAnimalsAvidinBinding, CompetitiveCalciumCattleDose-Response Relationship, DrugHumansIndicators and ReagentsKineticsLuminescenceModels, BiologicalMyoglobinRecombinant ProteinsSerum Albumin, BovineJournal Article

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BibTeXRIS

Maciej Adamczyk, Jeffrey A Moore, Kevin Shreder. 2002-02-11. Dual analyte detection using tandem flash luminescence.. https://doi.org/10.1016/s0960-894x(01)00762-4

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