PubMed · 1181482
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M Snook. 1975. Data-logging system for steady-state continuous flow analysers.. https://pubmed.ncbi.nlm.nih.gov/1181482/
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Modern hematology depends on rapid and accurate determination of quantitative and qualitative blood counts. Fully automated analyzers based on the principles of flow cytometry have replaced the traditional blood smear in the routine laboratory. They count the number of red cells, platelets and leukocytes with high precision. In addition, they divide the cells into different populations according to their physical or chemical properties. This population distribution pattern mimics a "differential" blood smear. Normality can be assessed with a high degree of accuracy, rapidly and reproducibly. The qualitative changes frequently provide pointers to the final diagnosis. In case of abnormalities the reports are flagged. A microscopic analysis should be added according to internal laboratory guidelines. It clarifies pathological findings and is essential in the search for specific abnormalities such as malaria. This underlines the need for exchange of information between physician and laboratory.
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Fast liquid chromatography was applied to the assay of several drugs in plasma. Short columns, 3.3-4 cm long, packed with C18 material, 3 microns particle size, were used. The peaks were little subject to extra-column band-broadening because the investigated drugs were eluted with high capacity factors in order to obtain an adequate separation from plasma components. The main influences on efficiency were the response time of the detector and the solvent composition of the injected sample. Conventional apparatus was used. A fully automated analytical system combining liquid-solid extraction via disposable extraction columns and fast liquid chromatography on a small-dimensioned 3 microns particle size column is described for the assay of drugs in plasma. Automation was accomplished by using the Automatic Sample Preparation with Extraction Columns system.