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PubMed · 11815849

Multilocus hybridization typing in Pediococcus acidilactici strains.

Diego Mora·Carlo Parini·Maria Grazia Fortina·Pier Luigi Manachini
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Abstract

In previous a study we demonstrated the presence of several genomic subpopulations within a collection of Pediococcus acidilactici strains isolated from different environments, through a multilocus typing analysis taking into consideration housekeeping conserved loci and protein coding genes of the primary metabolism. In this study, representative strains of five genomic subpopulations previously described (I, II, III, V, VII) were analyzed by restriction analysis of chromosomal DNA and subsequent hybridization assays using as probes amplified fragments obtained from five housekeeping genes (16S rDNA, rpoC, ldhD, ldhL, and metS). A computer similarity and clustering analysis of hybridization data showed the subdivision of P. acidilactici strains in five distinct genotypes according to the grouping previously obtained confirming that pediocin AcH/PA-1 producer strains represent one genomic lineage within the species P. acidilactici.

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5-Methyltetrahydrofolate-Homocysteine S-MethyltransferaseCluster AnalysisDNA ProbesDNA, BacterialDNA-Directed RNA PolymerasesL-Lactate DehydrogenaseNucleic Acid HybridizationPediococcusPhylogenyRNA, Ribosomal, 18SJournal Article

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Diego Mora, Carlo Parini, Maria Grazia Fortina, Pier Luigi Manachini. 2002. Multilocus hybridization typing in Pediococcus acidilactici strains.. https://doi.org/10.1007/s00284-001-0054-0

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