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Preparative chromatography of xylanase using expanded bed adsorption.

DosSantosEveraldo Silvino·Reginaldo Guirardello·Franco Telma Teixeira
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Abstract

Expanded bed adsorption was used to purify a marketable xylanase often used in the kraft pulp bleaching process. Experiments in packed and expanded beds were carried out mainly to study the adsorption of xylanase on to a cationic adsorbent (Streamline SP) in the presence of cells. In order to study the presence of cells, a Bacillus pumilus mass (5% wet mass) was mixed with the enzyme extract and submitted to an expanded bed adsorption system. One xylanase was purified to homogeneity in the packed bed. However, the 5% cell content hampered purification.

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AdsorptionChromatography, LiquidElectrophoresis, Polyacrylamide GelXylan Endo-1,3-beta-XylosidaseXylosidasesJournal ArticleResearch Support, Non-U.S. Gov't

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DosSantosEveraldo Silvino, Reginaldo Guirardello, Franco Telma Teixeira. 2002-01-25. Preparative chromatography of xylanase using expanded bed adsorption.. https://doi.org/10.1016/s0021-9673(01)01368-1

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