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Endothelin-A receptors and NO mediate decrease in arterial pressure during recovery from restraint.

Avery W C Yip·Teresa L Krukoff
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Abstract

We investigated the role of central endothelin-A (ET(A)) receptors and nitric oxide (NO) in regulating arterial pressure during restraint stress and recovery from stress. Rats received intracerebroventricular (icv) injections of the ET(A) receptor antagonist BQ123 (24 microg/kg) and were then subjected to two restraint-rest cycles (1 h of restraint and 1 h of rest/cycle). Although mean arterial pressure (MAP) values in BQ123-treated and control rats increased at the onset of restraint and remained elevated during restraint, MAP values in BQ123-treated rats were consistently greater than in control rats. During rest periods, MAP values in control rats decreased to below baseline levels, whereas those in BQ123-treated rats remained significantly higher. NO content was decreased in the brain stems of BQ123-treated compared with control rats after the 4-h protocol. Injections (icv) of the NO synthase (NOS) inhibitor N(G)-nitro-L-arginine (L-NNA) eliminated the decreases in MAP values during rest periods in both BQ123-treated and control rats. Inhibition of neuronal NOS with icv injection of 7-nitroindazole sodium salt resulted in MAP values intermediate between control rats and rats receiving L-NNA. These results support the hypothesis that endothelin acts through ET(A) receptors in the brain, possibly via release of NO, to decrease arterial pressure during restraint and recovery from restraint.

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AnimalsAntihypertensive AgentsBlood PressureEndothelin Receptor AntagonistsEnzyme InhibitorsIndazolesMaleNitric OxideNitric Oxide SynthaseNitric Oxide Synthase Type INitric Oxide Synthase Type IIINitroargininePeptides, CyclicRatsRats, Sprague-DawleyReceptor, Endothelin AReceptors, EndothelinRecovery of FunctionRestraint, PhysicalStress, PhysiologicalJournal ArticleResearch Support, Non-U.S. Gov't

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Avery W C Yip, Teresa L Krukoff. 2002. Endothelin-A receptors and NO mediate decrease in arterial pressure during recovery from restraint.. https://doi.org/10.1152/ajpregu.00308.2001

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