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Systemic RNAi in C. elegans requires the putative transmembrane protein SID-1.

William M Winston·Christina Molodowitch·Craig P Hunter
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Abstract

Double-stranded RNA-mediated gene interference (RNAi) in Caenorhabditis elegans systemically inhibits gene expression throughout the organism. To investigate how gene-specific silencing information is transmitted between cells, we constructed a strain that permits visualization of systemic RNAi. We used this strain to identify systemic RNA interference-deficient (sid) loci required to spread gene-silencing information between tissues but not to initiate or maintain an RNAi response. One of these loci, sid-1, encodes a conserved protein with predicted transmembrane domains. SID-1 is expressed in cells sensitive to RNAi, is localized to the cell periphery, and is required cell-autonomously for systemic RNAi.

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Amino Acid SequenceAnimalsAnimals, Genetically ModifiedCaenorhabditis elegansCaenorhabditis elegans ProteinsCalmodulin-Binding ProteinsCytoplasmEmbryo, NonmammalianGene SilencingGenes, HelminthGerm CellsGreen Fluorescent ProteinsIntestinal MucosaLuminescent ProteinsMembrane ProteinsMolecular Sequence DataMosaicismMuscle ProteinsMusclesMutationProtein Structure, TertiaryRNA, Double-StrandedRNA, HelminthRecombinant Fusion ProteinsTransgenesJournal ArticleResearch Support, Non-U.S. Gov'tResearch Support, U.S. Gov't, Non-P.H.S.

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William M Winston, Christina Molodowitch, Craig P Hunter. 2002-02-07. Systemic RNAi in C. elegans requires the putative transmembrane protein SID-1.. https://doi.org/10.1126/science.1068836

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