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PubMed · 11854485

An NMR approach to structural proteomics.

Adelinda Yee·Xiaoqing Chang·Antonio Pineda-Lucena·Bin Wu·Anthony Semesi·Brian Le·Theresa Ramelot·Gregory M Lee·Sudeepa Bhattacharyya·Pablo Gutierrez·Aleksej Denisov·Chang-Hun Lee·John R Cort·Guennadi Kozlov·Jack Liao·Grzegorz Finak·Limin Chen·David Wishart·Weontae Lee·Lawrence P McIntosh·Kalle Gehring·Michael A Kennedy·Aled M Edwards·Cheryl H Arrowsmith
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Abstract

The influx of genomic sequence information has led to the concept of structural proteomics, the determination of protein structures on a genome-wide scale. Here we describe an approach to structural proteomics of small proteins using NMR spectroscopy. Over 500 small proteins from several organisms were cloned, expressed, purified, and evaluated by NMR. Although there was variability among proteomes, overall 20% of these proteins were found to be readily amenable to NMR structure determination. NMR sample preparation was centralized in one facility, and a distributive approach was used for NMR data collection and analysis. Twelve structures are reported here as part of this approach, which allowed us to infer putative functions for several conserved hypothetical proteins.

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Bacterial ProteinsDatabases as TopicEscherichia coliMagnetic Resonance SpectroscopyModels, MolecularOpen Reading FramesProteinsSoftwareSpecies SpecificityJournal ArticleResearch Support, Non-U.S. Gov'tResearch Support, U.S. Gov't, Non-P.H.S.Research Support, U.S. Gov't, P.H.S.

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Adelinda Yee, Xiaoqing Chang, Antonio Pineda-Lucena, Bin Wu, Anthony Semesi, Brian Le, Theresa Ramelot, Gregory M Lee, Sudeepa Bhattacharyya, Pablo Gutierrez, Aleksej Denisov, Chang-Hun Lee, John R Cort, Guennadi Kozlov, Jack Liao, Grzegorz Finak, Limin Chen, David Wishart, Weontae Lee, Lawrence P McIntosh, Kalle Gehring, Michael A Kennedy, Aled M Edwards, Cheryl H Arrowsmith. 2002-02-19. An NMR approach to structural proteomics.. https://doi.org/10.1073/pnas.042684599

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