Array-based electrical detection of DNA with nanoparticle probes.

So-Jung Park; T Andrew Taton; Chad A Mirkin

doi:10.1126/science.1067003

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Array-based electrical detection of DNA with nanoparticle probes.

So-Jung Park·T Andrew Taton·Chad A Mirkin

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Abstract

A DNA array detection method is reported in which the binding of oligonucleotides functionalized with gold nanoparticles leads to conductivity changes associated with target-probe binding events. The binding events localize gold nanoparticles in an electrode gap; silver deposition facilitated by these nanoparticles bridges the gap and leads to readily measurable conductivity changes. An unusual salt concentration-dependent hybridization behavior associated with these nanoparticle probes was exploited to achieve selectivity without a thermal-stringency wash. Using this method, we have detected target DNA at concentrations as low as 500 femtomolar with a point mutation selectivity factor of approximately 100,000:1.

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Antigens, Bacterial Bacillus anthracis Bacterial Toxins Bacteriological Techniques DNA, Bacterial Electric Conductivity Electronics Gold Microelectrodes Nanotechnology Nucleic Acid Hybridization Oligonucleotide Array Sequence Analysis Oligonucleotide Probes Point Mutation Sensitivity and Specificity Silver Evaluation Study Journal Article Research Support, U.S. Gov't, Non-P.H.S.

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So-Jung Park, T Andrew Taton, Chad A Mirkin. 2002-02-22. Array-based electrical detection of DNA with nanoparticle probes.. https://doi.org/10.1126/science.1067003

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