Large-scale plant proteomics.

Birgit Kersten; Lukas Bürkle; Eckehard J Kuhn; Patrick Giavalisco; Zoltan Konthur; Angelika Lueking; Gerald Walter; Holger Eickhoff; Ulrich Schneider

PubMed · 11860206

Large-scale plant proteomics.

Birgit Kersten·Lukas Bürkle·Eckehard J Kuhn·Patrick Giavalisco·Zoltan Konthur·Angelika Lueking·Gerald Walter·Holger Eickhoff·Ulrich Schneider

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Abstract

Large-scale and high throughput approaches increasingly play an essential role in the study of biological systems, which are per se highly complex. Therefore, they need to be examined by these extensive methods to receive information about the large genomic and proteomic networks. In plant biology, this purpose has a strong support through the accessability of the complete genome sequence of the model plant Arabidopsis thaliana. This brief review intends to focus on the basics and the state-of-the-art of these high-throughput technologies and their application to plant proteomics. It describes protein microarrays, the use of antibodies, 2-DE and MS methods and the yeast two hybrid system, which are emerging as the major technologies for plant proteomics.

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Arabidopsis Electrophoresis, Gel, Two-Dimensional Genomics Mass Spectrometry Plants Proteome Two-Hybrid System Techniques Journal Article Review

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Birgit Kersten, Lukas Bürkle, Eckehard J Kuhn, Patrick Giavalisco, Zoltan Konthur, Angelika Lueking, Gerald Walter, Holger Eickhoff, Ulrich Schneider. 2002. Large-scale plant proteomics.. https://pubmed.ncbi.nlm.nih.gov/11860206/

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