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PubMed · 11877001

[Antigen loading on dendritic cells affects the cell function in stimulating T cells].

Y Wang·W Xie·Y Qiu·Z Gu·X Zhang
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Abstract

OBJECTIVE: To study the effect of antigen loading on dendritic cells (DC). METHODS: DCs collected from peripheral blood monocytes were loaded with a tumor antigen from XG-7 cell line. These DCs were then co-cultured with allogeneic T cells and were compared with those DCs without antigen exposure. RESULTS: Although DCs showed no change in their phenotypes after cultured with the antigen, they secreted more IL-12, and became more powerful in allogenic mixed lymphocyte reaction (MLR). Antigen-loaded DC stimulated more CD(4)(+) cells to proliferate than nonantigen-loaded DCs did. These CD(4)(+) cells did not kill XG-7 cells, but promoted CD(8)(+) cells' ability to inhibit the XG-7 proliferation. CONCLUSION: The proliferation of CD(4)(+) cells after cultured with DC may become an indicator for the function of antigen loaded DC and for the efficiency of DC immunotherapy.

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Antigens, NeoplasmCD4-Positive T-LymphocytesCD8-Positive T-LymphocytesCell DivisionCoculture TechniquesCytotoxicity, ImmunologicDendritic CellsEnzyme-Linked Immunosorbent AssayFlow CytometryHumansImmunophenotypingInterleukin-12T-LymphocytesTumor Cells, CulturedJournal ArticleResearch Support, Non-U.S. Gov't

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Y Wang, W Xie, Y Qiu, Z Gu, X Zhang. 2000. [Antigen loading on dendritic cells affects the cell function in stimulating T cells].. https://pubmed.ncbi.nlm.nih.gov/11877001/

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