NMR-based structural characterization of large protein-ligand interactions.

Maurizio Pellecchia; David Meininger; Qing Dong; Edcon Chang; Rick Jack; Daniel S Sem

doi:10.1023/a:1014256707875

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NMR-based structural characterization of large protein-ligand interactions.

Maurizio Pellecchia·David Meininger·Qing Dong·Edcon Chang·Rick Jack·Daniel S Sem

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Abstract

Genomic research on target identification and validation has created a great need for methods that rapidly provide detailed structural information on protein-ligand interactions. We developed a suite of NMR experiments as rapid and efficient tools to provide descriptive structural information on protein-ligand complexes. The methods work with large proteins and in particular cases also without the need for a complete three-dimensional structure. We will show applications with two tetrameric enzymes of 120 and 170 kDa.

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Binding Sites Combinatorial Chemistry Techniques Drug Design Ligands Models, Molecular Molecular Structure Nuclear Magnetic Resonance, Biomolecular Protein Binding Protein Conformation Proteins Journal Article

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Maurizio Pellecchia, David Meininger, Qing Dong, Edcon Chang, Rick Jack, Daniel S Sem. 2002. NMR-based structural characterization of large protein-ligand interactions.. https://doi.org/10.1023/a%3A1014256707875

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