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Normal-phase and stability-indicating reversed-phase high-performance liquid chromatographic methods for the determination of the novel antitumor agent: 1-methylpropyl-2-imidazolyldisulfide.

Ahmad Hashash·D Lynn Kirkpatrick·Merrill J Egorin·Lawrence H Block·John S Lazo
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Abstract

1-Methylpropyl-2-imidazolyl disulfide (MID) is a novel antitumor agent currently in Phase I clinical trials. The chromatographic behavior of MID and its potential impurity, degradation product, and metabolite 2-mercaptoimidazole (2MI) was studied under reversed-phase (RP) and normal-phase (NP) conditions. Both RP- and NP-HPLC separation methods were developed. RP-HPLC was validated as a stability-indicating assay for MID. NP-HPLC retained both MID and 2MI and pending further validation, could prove useful in the study of MID pharmacokinetics.

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Antineoplastic AgentsBuffersChromatography, High Pressure LiquidDisulfidesHumansHydrogen-Ion ConcentrationImidazolesReproducibility of ResultsSpectrophotometry, UltravioletJournal Article

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Ahmad Hashash, D Lynn Kirkpatrick, Merrill J Egorin, Lawrence H Block, John S Lazo. 2002-03-05. Normal-phase and stability-indicating reversed-phase high-performance liquid chromatographic methods for the determination of the novel antitumor agent: 1-methylpropyl-2-imidazolyldisulfide.. https://doi.org/10.1016/s1570-0232(01)00584-0

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