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Impaired T cell function in RANTES-deficient mice.

Yasuhiko Makino·Donald N Cook·Oliver Smithies·Olivia Y Hwang·Eric G Neilson·Laurence A Turka·Hiroshi Sato·Andrew D Wells·Theodore M Danoff
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Abstract

The chemokine RANTES is a chemoattractant for monocytes and T cells and is postulated to participate in many aspects of the immune response. To evaluate the biological roles of RANTES in vivo, we generated RANTES-deficient (-/-) mice and characterized their T cell function. In cutaneous delayed-type hypersensitivity assays, a 50% reduction in ear and footpad swelling was seen in -/- mice compared to +/+ mice. In vitro, polyclonal and antigen-specific T cell proliferation was decreased. Quantitative analysis using the fluorescent dye carboxy-fluorescein succinimidyl ester revealed that this proliferative defect was due both to fewer antigen-reactive T cells and to a reduction in the capacity of these cells to proliferate. In addition, IFN-gamma and IL-2 production by the -/- T cells was dramatically decreased. Together, these data suggest that RANTES is required for normal T cell functions as well as for recruiting monocytes and T cells to sites of inflammation.

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AnimalsBlotting, NorthernChemokine CCL5Flow CytometryHypersensitivity, DelayedInterferon-gammaInterleukin-2MiceT-LymphocytesJournal ArticleResearch Support, Non-U.S. Gov'tResearch Support, U.S. Gov't, P.H.S.

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Yasuhiko Makino, Donald N Cook, Oliver Smithies, Olivia Y Hwang, Eric G Neilson, Laurence A Turka, Hiroshi Sato, Andrew D Wells, Theodore M Danoff. 2002. Impaired T cell function in RANTES-deficient mice.. https://doi.org/10.1006/clim.2001.5178

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